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Maintenance of ELISA washer is treated in the relevant chapter. Basic maintenance frequency: daily Check that each channel optical sensors are clean. If dirt is detected, clean with a brush the surface of the Windows of the emitters of light and sensors. 2 Confirm that the lighting system is clean. Check that the calibration of the scanner is adequate. When starting the daily operations, allow the Analyzer to warm up for 30 minutes. Then, take a reading blank and then read a substrate-filled module. The readings must be identical.

If they are not, reverse module and repeat reading, in order to determine whether the deviation originates in the module or in the reader. 4 Examine the automatic feed of the plate. It should be smooth and consistent. Preventive maintenance frequency: quarterly 1. Check the stability of the lamp. Use the calibration filter, performing readings at intervals of 30 minutes or a plate. Compare the readings. There must be no differences. 2. Clean optical sensors systems and lighting systems. 3 Clean the plate advance mechanism. (Similarly see: American Society for Microbiology). 4. Check the alignment of each well with light emitters and detectors systems. ELISA BASIC DEFINITIONS. Developed technique to perform analysis for determining whether a substance is present in a sample. It is mainly used in the field of immunology. The word ELISA is an acronym for the words in the English language Enzyme-Linked Immunosorbent Assay. Enzyme. A protein that serves as a catalyst in a chemical reaction, accelerating the reactions. Fluorophore. Molecules that absorb light at a particular wavelength and emit light of a higher wavelength. ELISA washer. Equipment that is used for washing the plates during the stages of an ELISA test, in order to remove those components that have not joined in the reactions. ELISA washer uses special processes of washing buffers. Microplate reader. Name given to ELISA analyzers. ELISA plate. Item of consumption that has been standardized for the analysis by ELISA technique. The plates are generally 96 wells in a typical configuration of 8 rows by 12 columns. There are 384 ELISA plates and recently up 1 536 wells microplate, is have been imposing in centres of high demand, due to savings in supplies and reagents. Chemiluminescence. Light emission that appears during a chemical reaction. TMB. Used with the HRP enzyme substrate.