Journey For Hope

The break of my Mother country. " The mother country is happiness, pain and sky of all and not feudo nor chaplaincy of nadie." (Jose Mart) the word " Patria" it does not mean ground, nor laboratory of social experiments impelled by a dominant country. The word mother country agglutinates two basic representativenesses: first it aims inwards, that is to say: towards its settlers, the common coexistence under the inheritances, the customs, traditions, history, assumed from the childhood. Second evidently it is towards outside, that is to say the concert of the international community. Learn more at: Childrens Defense Fund. Then in a global world, already nobody is small barren island.

Doubt does not fit that, in a place where there is mother country, elements emphasize like: freedom, cooperating towards the communal property with loyalty and spirit on watch, everyone in the place that the destiny provided to him. The best mother country, without doubt is the one that respects the values morals, the one that it sustains to you, educates to you, gives health with quality, the one you that takes care of and guards by the surroundings, motivates to you towards the creativity and the critical thought, provides to you with stable-right sources of work and it lets to you live with the fruit of your effort. Mother country is not, to implement the theories of the chaos to benefit mine. Mother country is not to impose the brute force of villains who you envilece pester, you of taxes so that others are distributed gladly what you have won and of dessert they urge your children and costumary daughters to practice the perversion. Mother country is to prevail neither to run over all a nation, nor to say: " here control I and is made what I say cruel antojo". Mother country is not that the blood runs and is lightened, every day with deaths of women, men, children or adults all innocents or of people who raise their voices like flags.

Maintenance of ELISA washer is treated in the relevant chapter. Basic maintenance frequency: daily Check that each channel optical sensors are clean. If dirt is detected, clean with a brush the surface of the Windows of the emitters of light and sensors. 2 Confirm that the lighting system is clean. Check that the calibration of the scanner is adequate. When starting the daily operations, allow the Analyzer to warm up for 30 minutes. Then, take a reading blank and then read a substrate-filled module. The readings must be identical.

If they are not, reverse module and repeat reading, in order to determine whether the deviation originates in the module or in the reader. 4 Examine the automatic feed of the plate. It should be smooth and consistent. Preventive maintenance frequency: quarterly 1. Check the stability of the lamp. Use the calibration filter, performing readings at intervals of 30 minutes or a plate. Compare the readings. There must be no differences. 2. Clean optical sensors systems and lighting systems. 3 Clean the plate advance mechanism. (Similarly see: American Society for Microbiology). 4. Check the alignment of each well with light emitters and detectors systems. ELISA BASIC DEFINITIONS. Developed technique to perform analysis for determining whether a substance is present in a sample. It is mainly used in the field of immunology. The word ELISA is an acronym for the words in the English language Enzyme-Linked Immunosorbent Assay. Enzyme. A protein that serves as a catalyst in a chemical reaction, accelerating the reactions. Fluorophore. Molecules that absorb light at a particular wavelength and emit light of a higher wavelength. ELISA washer. Equipment that is used for washing the plates during the stages of an ELISA test, in order to remove those components that have not joined in the reactions. ELISA washer uses special processes of washing buffers. Microplate reader. Name given to ELISA analyzers. ELISA plate. Item of consumption that has been standardized for the analysis by ELISA technique. The plates are generally 96 wells in a typical configuration of 8 rows by 12 columns. There are 384 ELISA plates and recently up 1 536 wells microplate, is have been imposing in centres of high demand, due to savings in supplies and reagents. Chemiluminescence. Light emission that appears during a chemical reaction. TMB. Used with the HRP enzyme substrate.